Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed (ab98416)

Overview

  • Product nameGoat Anti-Rat IgG H&L (Cy3 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3 ®), pre-adsorbed
  • Target speciesRat
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details
  • ConjugationCy3 ®. Ex: 552nm, Em: 565nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab98416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/100 - 1/1000.
IHC-Fr 1/200.

added following abreview 37065

Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-EndoA alpha 1, 1:100
    Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody:Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3®) (ab98416), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained with SYTO 16

References for Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed (ab98416)

ab98416 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunohistochemistry (Frozen sections)
Goat polyclonal antibody to Rat IgG- H&L (Cy3®), ab98416, Lot: GR116866-2
Combined with anti-CD3 antibody (ab16669) and anti-CD19 antibodies (ab25232) and Goat anti-Rabbit IgG H&L (DyLight® 488) secondary antibody ab96895:

Following fixation in in 4% paramformaldehyde in PBS for 15 min, slides were blocked with 10% goat serum for 30 min and washed 3 times with ice-cold PBS, and incubated with mixture of anti-CD3 (ab16669) and anti-CD19 antibodies (ab25232), diluted 1:200 in PBST (1× PBS with 0.05% Tween-20), 1% BSA for 1 hour in a humidified chamber at room temperature.
Slides were washed 4 times in PBS (5 min each) and incubated for 1 hour in dark at room temperature with mixture of secondary antibodies: Goat DyLight® 488-conjugated anti-Rabbit (ab96895) and Goat Cy3®-conjugated antibody to Rat IgG - H&L (ab98416), diluted 1:200 in PBST, 1% BSA.
Slides were washed 3 times with PBS in dark (each wash for 5 min) and counterstained with 0.1 µg/ml DAPI for 5 min and finally rinsed with PBS, left 10 minutes for air-drying in dark and finally mounted.
CD3 antigen appears green CD19 red and the nuclei are stained blue.
Fluorescent microscope pictures at 10x.
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Herr Dr. Nasr Hemdan

Verified customer

Submitted Jul 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"