Overview

  • Product name
    Goat Anti-Rat IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Target species
    Rat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Jones CI  et al. RNA-seq reveals post-transcriptional regulation of Drosophila insulin-like peptide dilp8 and the neuropeptide-like precursor Nplp2 by the exoribonuclease Pacman/XRN1. Nucleic Acids Res 44:267-80 (2016). ICC/IF . Read more (PubMed: 26656493) »
  • Lundholm L  et al. Resistance to DNA-damaging treatment in non-small cell lung cancer tumor-initiating cells involves reduced DNA-PK/ATM activation and diminished cell cycle arrest. Cell Death Dis 4:e478 (2013). Flow Cyt ; Human . Read more (PubMed: 23370278) »

See all 2 Publications for this product

Customer reviews and Q&As

Hello!

The background staining may be due to detection of endogenous immunoglobulins by secondary. I suggest pre blocking the section with unconjugated anti-rat antibodies (or anti mouse if its mouse tissue) to reduce this background. I also s...

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The concentration is 0.5 mg/ml and is provided in a volume of 1 ml.

I hope this is helpful. Please contact us again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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