The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200.
1/50 - 1/500.
1/50 - 1/500.
DyLight® - Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab98420)
Emission spectra of DyLight® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab98420)
ICC/IF image of ab6161 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rat- H&L, pre-adsorbed (ab98420) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
Shrestha RL et al. TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes. Open Biol4:130108 (2014).
Read more (PubMed: 24898139) »
Liegel RP et al. Loss-of-function mutations in TBC1D20 cause cataracts and male infertility in blind sterile mice and Warburg micro syndrome in humans. Am J Hum Genet93:1001-14 (2013).
Read more (PubMed: 24239381) »