Goat Anti-Rat IgG H&L (HRP) (ab6845)
Key features and details
- Goat Anti-Rat IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
Overview
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Product name
Goat Anti-Rat IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Tested applications
Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
Immunogen
Rat IgG whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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ELISA |
Use at an assay dependent dilution.
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Immunomicroscopy |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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ICC/IF |
1/500 - 1/1500.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent dilution. |
ELISA
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/500 - 1/1500. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (10)
ab6845 has been referenced in 10 publications.
- Drwesh L et al. A network of cytosolic (co)chaperones promotes the biogenesis of mitochondrial signal-anchored outer membrane proteins. Elife 11:N/A (2022). PubMed: 35876647
- Brioudes F et al. HASTY, the Arabidopsis EXPORTIN5 ortholog, regulates cell-to-cell and vascular microRNA movement. EMBO J 40:e107455 (2021). PubMed: 34152631
- Park J et al. The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins. Nucleic Acids Res 49:12517-12534 (2021). PubMed: 34850140
- Ryu I et al. eIF4A3 Phosphorylation by CDKs Affects NMD during the Cell Cycle. Cell Rep 26:2126-2139.e9 (2019). PubMed: 30784594
- Kittipassorn T et al. Characterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1. Exp Eye Res 181:127-135 (2019). PubMed: 30677389
- Funk N et al. The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4. Sci Rep 9:4515 (2019). PubMed: 30872638
- Jores T et al. Cytosolic Hsp70 and Hsp40 chaperones enable the biogenesis of mitochondrial ß-barrel proteins. J Cell Biol 217:3091-3108 (2018). PubMed: 29930205
- Vitali DG et al. Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes. Elife 7:N/A (2018). PubMed: 29923829
- Schneberger D et al. Toll-like receptor 9 partially regulates lung inflammation induced following exposure to chicken barn air. J Occup Med Toxicol 11:31 (2016). IHC-P . PubMed: 27375768
- Lesch HP et al. Avidin fusion protein-expressing lentiviral vector for targeted drug delivery. Hum Gene Ther 20:871-82 (2009). IHC-FoFr . PubMed: 19419273