Overview

  • Product name
    Goat Anti-Rat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target species
    Rat
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1500.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

References for Goat Anti-Rat IgG H&L (HRP) (ab6845)

This product has been referenced in:
  • Schneberger D  et al. Toll-like receptor 9 partially regulates lung inflammation induced following exposure to chicken barn air. J Occup Med Toxicol 11:31 (2016). IHC-P . Read more (PubMed: 27375768) »
  • Lesch HP  et al. Avidin fusion protein-expressing lentiviral vector for targeted drug delivery. Hum Gene Ther 20:871-82 (2009). IHC-FoFr . Read more (PubMed: 19419273) »

See all 2 Publications for this product

Product Wall

Thank you for your reply.

The secondary ab98385 is a similar product: goat anti-rat IgG H&L conjugated to biotin and pre-adsorbed. This antibody shows minimal cross-reactivity to mouse and human IgGs. I hope this helps, please let me know...

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Thank you for contacting us.

The secondary antibody ab6845 has not been pre-adsorbed or tested for reactivity with mouse IgG. I would recommend the similar antibody ab98391, which has been pre-adsorbed and shows < 1% cross-reactivity to hu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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