• Product name
    Goat Anti-Rat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
  • Target species
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
  • Isotype
  • General notes
    Part of the AbExcel range.
  • Research areas


Our Abpromise guarantee covers the use of ab97057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (as primary)
WB 1/2000 - 1/20000. Colorimetric: Use 1/5000-1/30000, Chemiluminescent: use 1/10000-1/50000


  • IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97057, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml

    Lanes 1-6:  HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab97057) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab97057) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab97057) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 52 kDa

    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.



This product has been referenced in:
  • Tabet R  et al. CUG initiation and frameshifting enable production of dipeptide repeat proteins from ALS/FTD C9ORF72 transcripts. Nat Commun 9:152 (2018). Read more (PubMed: 29323119) »
  • Zhou Q  et al. Ultrasound combined with targeted cationic microbubble-mediated angiogenesis gene transfection improves ischemic heart function. Exp Ther Med 13:2293-2303 (2017). Read more (PubMed: 28565841) »

See all 14 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
The secondary ab works well. Based on my experience for IHC the dilution factor requires optimization based on the tissue and antigen retrieval method used.
In the attached picture you can see F480 used as primary antibody and the secondary Goat Anti-Rat IgG H&L ab 97057 used at the concentration of 1:200.
The dilution 1:500 works well too.

Dr. Francesco Elia Marino

Verified customer

Submitted Apr 15 2016

Western blot
The price of this antibody is good based on the amount provided with the shipment.
I used this Antibody at the dilution 1:10000 and it worked fine in my WB.
In the attached picture ab6640 was used as primary antbody in tissue lysates from human macrophages and ab 97057 was used as secondary antibody. The secondary and primary antibodies were diluted in 2% Milk in PBS.

Dr. Francesco Elia Marino

Verified customer

Submitted Apr 15 2016

Western blot
We are using an 2 years old vial of this antibody, it still works well.

Abcam user community

Verified customer

Submitted Nov 05 2015

Western blot
Antibody recognizes the Rat antibody specifically. I analyzed an HA tagged protein (0.4 OD cells equivalent) and used the anti-Rat HA antibody that was purchased from Roche and this secondary antibody ab97057 was used as secondary. I used 1:10K dilution of the antibody and incubated for 1hr at room temperature. Nitrocellulose membrane was used for blotting. On the blot the lane 1, 2, 3 were three individual clones picked after transformation. The protein of the right molecular weight was observed. (70kD).

Abcam user community

Verified customer

Submitted Mar 26 2014

Western blot
Lane 1 is wild-type mouse ES cells expressing E-cadherin. Lane 2 is an Ecad-/- mES cell line. DECMA-1 primary antibody used (not Abcam). Secondary used at 1:5000 for 1 hour. Use of secondary at higher concentration of 1:2000 results in background staining.

Abcam user community

Verified customer

Submitted Nov 28 2013

Thank you for your reply. I am sorry that these antibodies did not perform as stated on the datasheet. It is regrettable these antibodies have not worked even though replacements have been tried.

As requested, I have asked our Finance depart...

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Thank you for your message.

I am sincerely sorry to hear this is still not working for you.

I am pleased to raise a credit note for the two items originally purchased. This can be used to pay towards the purchase of any other items ...

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Thank you for confirming these details and for your cooperation.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge alternative replaceme...

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Thank you for your reply.

Any anti rat IgG antibody tested in IHC-P should be suitable for your experiments and would be covered by the guarantee. I can suggest you may like to try the following. I can recommend to review the online datashee...

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Thank you for your message.

It is indeed disappointing that the results are not yet satisfactory, it is regrettably and I appreciate your concerns.

In this case, I would suggest it would be most beneficial to try a different secon...

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