Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)

Overview

  • Product nameGoat Anti-Rat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP), pre-adsorbed
  • Target speciesRat
  • Tested applicationsICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot Blotmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Full length native Rat IgG (purified).

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1500.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ELISA 1/50000.
Dot Blot Use at an assay dependent dilution.

Goat Anti-Rat IgG H&L (HRP) preadsorbed images

  • Whole mount immunostaining of E11.5 mouse embryo  (Blood vessels in the embryonic head). This staining was performed with rat monoclonal antibody to Pecam followed by staining with ab 7097 (HRP conjugated).

    This picture was kindly supplied as part of the review submitted by Helen Makarenkova.

References for Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)

This product has been referenced in:
  • Workman AM  et al. Inflammation enhances IL-2 driven differentiation of cytolytic CD4 T cells. PLoS One 9:e89010 (2014). WB ; Mouse . Read more (PubMed: 24586481) »
  • Vaeth M  et al. Follicular regulatory T cells control humoral autoimmunity via NFAT2-regulated CXCR5 expression. J Exp Med 211:545-61 (2014). Read more (PubMed: 24590764) »

See all 3 Publications for this product

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Please reffer the customer to our FAQs and our Storage guide:

"For many of our antibodies, freezing at -20oC or -80oC in small aliquots is the optimal storage condition. Aliquotting minimizes damag...

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As requested, I have issued a free of charge replacement for ab7097, anti-rat IgG cross-absorbed to remove reactivity with mouse IgG. I apologize for the misunderstanding on our part and wish you the best of luck with your research.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"