Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)

Overview

  • Product name
    Goat Anti-Rat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rat
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blotmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Full length native Rat IgG (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1500.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ELISA 1/50000.
Dot blot Use at an assay dependent dilution.

Images

  • Whole mount immunostaining of E11.5 mouse embryo  (Blood vessels in the embryonic head). This staining was performed with rat monoclonal antibody to Pecam followed by staining with ab 7097 (HRP conjugated).

    This picture was kindly supplied as part of the review submitted by Helen Makarenkova.

References

This product has been referenced in:
  • Wang J  et al. Regulation of type II collagen, matrix metalloproteinase-13 and cell proliferation by interleukin-1ß is mediated by curcumin via inhibition of NF-?B signaling in rat chondrocytes. Mol Med Rep 16:1837-1845 (2017). Read more (PubMed: 28627596) »
  • Ratanabanangkoon K  et al. A novel in vitro potency assay of antisera against Thai Naja kaouthia based on nicotinic acetylcholine receptor binding. Sci Rep 7:8545 (2017). Read more (PubMed: 28819275) »

See all 9 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Please reffer the customer to our FAQs and our Storage guide:

"For many of our antibodies, freezing at -20oC or -80oC in small aliquots is the optimal storage condition. Aliquotting minimizes damag...

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As requested, I have issued a free of charge replacement for ab7097, anti-rat IgG cross-absorbed to remove reactivity with mouse IgG. I apologize for the misunderstanding on our part and wish you the best of luck with your research.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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