Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab98425)
Key features and details
- Goat Anti-Rat IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
Overview
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Product name
Goat Anti-Rat IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Tested applications
Suitable for: IHC-P, ELISA, WB, ICCmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/5000.
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|
ELISA |
1/10000 - 1/100000. (as Primary).
|
|
WB |
1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000.
|
|
ICC |
Use at an assay dependent dilution.
|
Notes |
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IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. (as Primary). |
WB
1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000. |
ICC
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab98425 has been referenced in 4 publications.
- Iida Y et al. AMIGO2 expression as a predictor of recurrence in cervical cancer with intermediate risk. Mol Clin Oncol 19:56 (2023). PubMed: 37323247
- Izutsu R et al. AMIGO2 contained in cancer cell-derived extracellular vesicles enhances the adhesion of liver endothelial cells to cancer cells. Sci Rep 12:792 (2022). PubMed: 35039535
- Goto K et al. Establishment of an antibody specific for AMIGO2 improves immunohistochemical evaluation of liver metastases and clinical outcomes in patients with colorectal cancer. Diagn Pathol 17:16 (2022). PubMed: 35094710
- Goto K et al. The impact of AMIGO2 on prognosis and hepatic metastasis in gastric cancer patients. BMC Cancer 22:280 (2022). PubMed: 35296279