Goat anti-Rat IgG H&L (PE) secondary antibody (ab7010)

Overview

  • Product nameGoat anti-Rat IgG H&L (PE) secondary antibody
    See all IgG secondary antibodies
  • Target speciesRat
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Full length native Rat IgG (purified).

  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250.

Use 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.

References for Goat anti-Rat IgG H&L (PE) secondary antibody (ab7010)

This product has been referenced in:
  • Zhang ZH  et al. An integrated lipidomics and metabolomics reveal nephroprotective effect and biochemical mechanism of Rheum officinale in chronic renal failure. Sci Rep 6:22151 (2016). IHC-P . Read more (PubMed: 26903149) »
  • Kim SM  et al. Acquired resistance to cetuximab is mediated by increased PTEN instability and leads cross-resistance to gefitinib in HCC827 NSCLC cells. Cancer Lett 296:150-9 (2010). Flow Cyt . Read more (PubMed: 20444542) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry and for bringing this matter to my attention. A mistake appears to have occurred on our product data sheet and the value should read ug and not mg. I have updated the product data sheet to reflect this change. I apologise for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"