Goat Anti-Rat IgM mu chain (DyLight® 488) preadsorbed (ab98368)
- Product nameGoat Anti-Rat IgM mu chain (DyLight® 488) preadsorbedSee all IgM secondary antibodies ...
- DescriptionGoat polyclonal Secondary Antibody to Rat IgM - mu chain (DyLight® 488), pre-adsorbed
- Target speciesRat
- Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
Human, MouseTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
- ConjugationDyLight® 488. Ex: 493nm, Em: 518nm
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab98368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: 1/50 - 1/500.|
|ICC/IF||ICC/IF: 1/50 - 1/500.|
|Flow Cyt||Flow Cyt: 1/50 - 1/200.|
Goat Anti-Rat IgM mu chain (DyLight® 488) preadsorbed images
Overlay histogram showing THP1 cells stained with ab16081 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16081, 1µg/1x10^6 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgM (mu chain) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Rat IgM [RTK2118] (ab35768, 2µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
References for Goat Anti-Rat IgM mu chain (DyLight® 488) preadsorbed (ab98368)
ab98368 has not yet been referenced specifically in any publications.