For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
GOLD antibody purification kit (ab204909) removes common buffer constituents found in commercial antibodies (e.g. BSA, glycine, tris and azide) that decrease the efficiency of conjugation reactions with GOLD particles using our GOLD conjugation kits. ab204909 can also be used to purify antibodies from crude samples such as ascites fluid or serum. The antibody should ideally be in a volume of 100 - 500 µl. Up to 500 µg of antibody can be purified in each run.
The method involves capture of the antibody on Protein A Resin and removal of unwanted substances by a simple wash procedure, using a standard microfuge. The purified antibody is then eluted and neutralized.
Antibodies purified using the GOLD antibody purification kit are fully compatible with our GOLD antibody conjugation kits. To purify antibodies for use with our other antibody conjugation kits, we recommend using one of our other purification kits - view our selection guide.
For purifying mouse IgG, we recommend using GOLD mouse antibody purification kit ab204910.
|Components||1 tests||3 tests|
|10X Binding Buffer||1 vial||1 vial|
|Additional Collecting Tubes||4 vials||12 vials|
|Elution Buffer||1 vial||1 vial|
|Neutralizer||1 vial||1 vial|
|Protein A resin||1 vial||3 vials|
|Spin cartridge/ collecting tube assemblies||1 unit||3 units|
|Wash Buffer||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab204909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|AP||Use at an assay dependent concentration.|
ab204909 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"