Overview

  • Product name
  • Description
    Goat polyclonal to GOLGA3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-HSQSRASKEGPGE

    , corresponding to C terminal amino acids 1485-1498 of Human GOLGA3 (according to NP_005886.2).

  • Positive control
    • Human HeLa cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 167 kDa).

Target

  • Relevance
    GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.
  • Cellular localization
    Cytoplasmic and Golgi Apparatus
  • Database links
  • Alternative names
    • GCP170 antibody
    • GOLGA 3 antibody
    • GOLGA3 antibody
    • Golgi autoantigen antibody
    • Golgi complex associated protein of 170 kDa antibody
    • Golgi complex-associated protein of 170 kD antibody
    • Golgi membrane associated protein antibody
    • Golgi peripheral membrane protein antibody
    • Golgin 160 antibody
    • Golgin subfamily a 3 antibody
    • HGNC:4426 antibody
    • Male enhanced antigen 2 antibody
    • MEA2 antibody
    • SY2/SY10 protein antibody
    see all

Images

  • Anti-GOLGA3 antibody (ab40837) at 0.1 µg/ml + Hela lysate at 35 µg

    Secondary
    anti-goat-HRP 1:3000 Dilution

    Predicted band size : 167 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Jelínek M  et al. Caspase-2 is involved in cell death induction by taxanes in breast cancer cells. Cancer Cell Int 13:42 (2013). WB . Read more (PubMed: 23672670) »
  • Clarke JH  et al. Localization of phosphatidylinositol phosphate kinase IIgamma in kidney to a membrane trafficking compartment within specialized cells of the nephron. Am J Physiol Renal Physiol 295:F1422-30 (2008). WB ; Human . Read more (PubMed: 18753295) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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