Overview

  • Product name
  • Description
    Rabbit polyclonal to GOLGA3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1436 and 1498 of GOLGA3 (NP_005886).

  • Positive control
    • 293T, A431, H1299 cell lysates for WB and HeLa cells for IF

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 167 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Relevance
    GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.
  • Cellular localization
    Cytoplasmic and Golgi Apparatus
  • Database links
  • Alternative names
    • GCP170 antibody
    • GOLGA 3 antibody
    • GOLGA3 antibody
    • Golgi autoantigen antibody
    • Golgi complex associated protein of 170 kDa antibody
    • Golgi complex-associated protein of 170 kD antibody
    • Golgi membrane associated protein antibody
    • Golgi peripheral membrane protein antibody
    • Golgin 160 antibody
    • Golgin subfamily a 3 antibody
    • HGNC:4426 antibody
    • Male enhanced antigen 2 antibody
    • MEA2 antibody
    • SY2/SY10 protein antibody
    see all

Images

  • All lanes : Anti-GOLGA3 antibody (ab96080) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 167 kDa



    5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96080 at 1/200 dilution. Lower image merged with DNA probe.

References

This product has been referenced in:
  • Turowec JP  et al. An unbiased proteomic screen reveals caspase cleavage is positively and negatively regulated by substrate phosphorylation. Mol Cell Proteomics 13:1184-97 (2014). WB ; Human . Read more (PubMed: 24556848) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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