Overview

  • Product name
  • Description
    Rabbit polyclonal to GOLGA5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 237-566 of Human GOLGA5 (Uniprot ID Q8TBA6)

  • Positive control
    • A549 and Mouse brain whole cell lysates, methanol-fixed HeLa cells, paraffin-embedded Human smooth muscle tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.
  • Tissue specificity
    Ubiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa.
  • Involvement in disease
    Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving GOLGA5 is found in thyroid papillary carcinomas. Translocation t(10;14)(q11;q32) with RET. The translocation generates the RET/GOLGA5 (PTC5) oncogene which was found in 2 cases of PACT in children exposed to radioactive fallout after Chernobyl.
  • Post-translational
    modifications
    Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase.
  • Cellular localization
    Golgi apparatus membrane. Found throughout the Golgi, both on cisternae and, at higher abundance, on the tubulo-vesicular structures of the cis-Golgi network.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 31 antibody
    • Cell proliferation-inducing gene 31 protein antibody
    • GOGA5_HUMAN antibody
    • GOLGA 5 antibody
    • GOLGA5 antibody
    • Golgi autoantigen golgin subfamily a 5 antibody
    • Golgi integral membrane protein 5 antibody
    • Golgin 84 antibody
    • Golgin A5 antibody
    • Golgin subfamily A member 5 antibody
    • Golgin-84 antibody
    • Golgin84 antibody
    • GOLIM 5 antibody
    • GOLIM5 antibody
    • Protein Ret-II antibody
    • PTC5 antibody
    • Ret fused gene 5 antibody
    • RET fused gene 5 protein antibody
    • Ret II antibody
    • Ret II protein antibody
    • RET-fused gene 5 protein antibody
    • RFG 5 antibody
    • RFG5 antibody
    see all

Images

  • Anti-GOLGA5 antibody (ab155806) at 1/1000 dilution + A459 whole cell lysate at 30 µg

    Predicted band size: 83 kDa



    7.5% SDS PAGE
  • Anti-GOLGA5 antibody (ab155806) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 83 kDa



    7.5% SDS PAGE
  • Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling GOLGA5 with ab155806 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an anti-alpha-tubulin antibody at 1/2500 (red).
  • Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue, labeling GOLGA5 with ab155806 at 1/100 dilution.

References

ab155806 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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