Overview

  • Product nameAnti-GOLGA5 antibody
    See all GOLGA5 primary antibodies
  • Description
    Mouse polyclonal to GOLGA5
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human GOLGA5, amino acids 1-731 (NP_005104.2)

  • Positive control
    • Human liver tisssue lysate, HepG2 cell lysate, GOLGA5 transfected 293T cell lysate and HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionInvolved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.
  • Tissue specificityUbiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa.
  • Involvement in diseaseDefects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving GOLGA5 is found in thyroid papillary carcinomas. Translocation t(10;14)(q11;q32) with RET. The translocation generates the RET/GOLGA5 (PTC5) oncogene which was found in 2 cases of PACT in children exposed to radioactive fallout after Chernobyl.
  • Post-translational
    modifications
    Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase.
  • Cellular localizationGolgi apparatus membrane. Found throughout the Golgi, both on cisternae and, at higher abundance, on the tubulo-vesicular structures of the cis-Golgi network.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing gene 31 antibody
    • Cell proliferation-inducing gene 31 protein antibody
    • GOGA5_HUMAN antibody
    • GOLGA 5 antibody
    • GOLGA5 antibody
    • Golgi autoantigen golgin subfamily a 5 antibody
    • Golgi integral membrane protein 5 antibody
    • Golgin 84 antibody
    • Golgin A5 antibody
    • Golgin subfamily A member 5 antibody
    • Golgin-84 antibody
    • Golgin84 antibody
    • GOLIM 5 antibody
    • GOLIM5 antibody
    • Protein Ret-II antibody
    • PTC5 antibody
    • Ret fused gene 5 antibody
    • RET fused gene 5 protein antibody
    • Ret II antibody
    • Ret II protein antibody
    • RET-fused gene 5 protein antibody
    • RFG 5 antibody
    • RFG5 antibody
    see all

Anti-GOLGA5 antibody images

  • Anti-GOLGA5 antibody (ab89136) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • Anti-GOLGA5 antibody (ab89136) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • All lanes : Anti-GOLGA5 antibody (ab89136) at 1 µg/ml

    Lane 1 : GOLGA5 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • ab89136 at 10µg/ml staining GOLGA5 in HeLa cells by Immunofluorescence.

References for Anti-GOLGA5 antibody (ab89136)

ab89136 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"