Overview

  • Product name
  • Description
    Rabbit polyclonal to Golgin 97
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 18-67 (RPGGATRIPR SVSKESVASM GADSGDDFAS DGSSSREDLS SQLLRRNEQI) of Human Golgin 97 (NP_002068).

  • Positive control
    • HCT15 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituents: 98% PBS, 2% Sucrose
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Affinity purifications with biochemical fractionation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 88 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-Golgin 97 antibody (ab112084) at 1 µg/ml + HCT15 cell lysate at 10 µg

    Predicted band size : 88 kDa

References

ab112084 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole heart lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-15%)
Loading amount
30 µg
Specification
Whole heart lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up