Overview

  • Product nameAnti-GOLPH2 antibody [6C9]
    See all GOLPH2 primary antibodies
  • Description
    Mouse monoclonal [6C9] to GOLPH2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant full length protein of Human GOLPH2 produced in HEK293T cells (NP_808800).

  • Positive control
    • HEK293T cells transfected with GOLPH2, HeLa, A549 and COS7 cell extract, Adenocarcinoma of Human ovary, Carcinoma of Human thyroid, Human endometrium, Adenocarcinoma of Human endometrium, Human prostate and Carcinoma of Human bladder tissues, COS7 cells transiently transfected with GOLPH2
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab119800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 45 kDa.
IHC-P 1/150.
ICC/IF 1/100.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionUnknown. Cellular response protein to viral infection.
  • Tissue specificityWidely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.
  • Sequence similaritiesBelongs to the GOLM1/CASC4 family.
  • Post-translational
    modifications
    Glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationGolgi apparatus > cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA379P1.3 antibody
    • C9orf155 antibody
    • Chromosome 9 open reading frame 155 antibody
    • Golgi membrane protein 1 antibody
    • Golgi membrane protein GP73 antibody
    • Golgi phosphoprotein 2 antibody
    • Golgi protein 73 kD antibody
    • Golgi protein 73kD antibody
    • GOLM 1 antibody
    • GOLM1 antibody
    • GOLM1_HUMAN antibody
    • GOLPH 2 antibody
    • GOLPH2 antibody
    • GP 73 antibody
    • GP73 antibody
    • PSEC0257 antibody
    see all

Anti-GOLPH2 antibody [6C9] images

  • Overlay histogram showing HeLa cells stained with ab119800 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119800, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-GOLPH2 antibody [6C9] (ab119800) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY GOLPH2

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 45 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200086)
  • All lanes : Anti-GOLPH2 antibody [6C9] (ab119800) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SVT2 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : COS7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MDCK cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : MCF7 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 45 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200086)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using ab119800 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using ab119800 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human endometrium tissue using ab119800 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using ab119800 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human prostate tissue using ab119800 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using ab119800 at a dilution of 1/150.
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GOLPH2 using ab119800 at a dilution of 1/100.

References for Anti-GOLPH2 antibody [6C9] (ab119800)

ab119800 has not yet been referenced specifically in any publications.

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