Overview

  • Product nameAnti-GOLPH2 antibody
    See all GOLPH2 primary antibodies
  • Description
    Goat polyclonal to GOLPH2
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-NLLDQREKRNHTL

    , corresponding to C terminal amino acids 389-401 of Human GOLPH2

  • Positive control
    • Human small intestine tissue, human prostate tissue, human uterus tissue, HEK293 lysate overexpressing Human GOLM1 with C-terminal MYC tag.

Properties

Applications

Our Abpromise guarantee covers the use of ab110008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 45 kDa.
ELISA 1/16000.
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • FunctionUnknown. Cellular response protein to viral infection.
  • Tissue specificityWidely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.
  • Sequence similaritiesBelongs to the GOLM1/CASC4 family.
  • Post-translational
    modifications
    Glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationGolgi apparatus > cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA379P1.3 antibody
    • C9orf155 antibody
    • Chromosome 9 open reading frame 155 antibody
    • Golgi membrane protein 1 antibody
    • Golgi membrane protein GP73 antibody
    • Golgi phosphoprotein 2 antibody
    • Golgi protein 73 kD antibody
    • Golgi protein 73kD antibody
    • GOLM 1 antibody
    • GOLM1 antibody
    • GOLM1_HUMAN antibody
    • GOLPH 2 antibody
    • GOLPH2 antibody
    • GP 73 antibody
    • GP73 antibody
    • PSEC0257 antibody
    see all

Anti-GOLPH2 antibody images

  • ab110008, at 3.75 µg/ml, staining GOLPH2 in Human small intestine tissue by immunohistochemistry.
  • ab110008, at 3.75 µg/ml, staining GOLPH2 in Human prostate tissue by immunohistochemistry.
  • ab110008, at 3.75 µg/ml, staining GOLPH2 in Human uterus tissue by immunohistochemistry.
  • Lane 1 : Anti-GOLPH2 antibody (ab110008) at 1 µg/ml
    Lane 2 : Anti-GOLPH2 antibody (ab110008) at 1 mg/ml
    Lane 3 : anti-Myc tag at 1/1000 dilution

    Lane 1 : HEK293 lysate (in RIPA buffer) overexpressing Human GOLM1 with C-terminal MYC tag at 10 µg
    Lane 2 : Mock-transfected HEK293
    Lane 3 : HEK293 lysate (in RIPA buffer) overexpressing Human GOLM1 with C-terminal MYC tag at 10 µg


    Predicted band size : 45 kDa

References for Anti-GOLPH2 antibody (ab110008)

ab110008 has not yet been referenced specifically in any publications.

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