Overview

  • Product name
  • Description
    Rabbit polyclonal to GOLPH3
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to the N terminal of Human GOLPH3.

  • Positive control
    • Human prostate tissue; Rat lung tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab113649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
  • Tissue specificity
    Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
  • Sequence similarities
    Belongs to the GOLPH3/VPS74 family.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coat protein antibody
    • Coat protein GPP34 antibody
    • FLJ90675 antibody
    • Golgi associated protein antibody
    • Golgi peripheral membrane protein 1, 34 kDa antibody
    • Golgi phosphoprotein 3 (coat protein) antibody
    • Golgi phosphoprotein 3 antibody
    • Golgi protein antibody
    • GOLP3_HUMAN antibody
    • Golph3 antibody
    • GOPP1 antibody
    • GPP34 antibody
    • MIDAS antibody
    • Mitochondrial DNA absence factor antibody
    see all

Images

  • ab113649, at 5 µg/ml, staining GOLPH3 in Formalin fixed, Paraffin embedded Human prostate tissue by Immunohistochemistry.
  • Lane 1 : Anti-GOLPH3 antibody (ab113649) at 0.5 µg/ml
    Lane 2 : Anti-GOLPH3 antibody (ab113649) at 1 µg/ml

    Lane 1 : Rat lung tissue lysate
    Lane 2 : Rat lung tissue lysate


    Predicted band size : 34 kDa

References

ab113649 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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