IHC-P human prostate carcinoma stained with ab82377, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot - GOLPH3 antibody - N-terminal (ab82377)
Anti-GOLPH3 antibody (ab82377) at 1/50 dilution + Hela cell line lysates at 35 µg
Predicted band size : 34 kDa Observed band size : 34 kDa Additional bands at : 31 and 41 kDa,54 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometry - Anti-GOLPH3 antibody (ab82377)
Flow cytometric analysis of hela cells using ab82377 (bottom) compared to a negative control cell (top). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
References for Anti-GOLPH3 antibody (ab82377)
This product has been referenced in:
Tokuda E et al. Phosphatidylinositol 4-phosphate in the Golgi apparatus regulates cell-cell adhesion and invasive cell migration in human breast cancer. Cancer Res74:3054-66 (2014).
Read more (PubMed: 24706697) »
Isaji T et al. An Oncogenic Protein Golgi Phosphoprotein 3 Up-regulates Cell Migration via Sialylation. J Biol Chem289:20694-20705 (2014).
Read more (PubMed: 24895123) »