Overview

  • Product nameAnti-GOLPH3 antibody
    See all GOLPH3 primary antibodies
  • Description
    Rabbit polyclonal to GOLPH3
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    a 14 amino acid peptide from near the amino terminus of human GOLPH3 (AAH12123).

  • Positive control
    • Rat Lung Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/150.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionInvolved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
  • Tissue specificityDetected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
  • Sequence similaritiesBelongs to the GOLPH3/VPS74 family.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationCell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coat protein antibody
    • Coat protein GPP34 antibody
    • FLJ90675 antibody
    • Golgi associated protein antibody
    • Golgi peripheral membrane protein 1, 34 kDa antibody
    • Golgi phosphoprotein 3 (coat protein) antibody
    • Golgi phosphoprotein 3 antibody
    • Golgi protein antibody
    • GOLP3_HUMAN antibody
    • Golph3 antibody
    • GOPP1 antibody
    • GPP34 antibody
    • MIDAS antibody
    • Mitochondrial DNA absence factor antibody
    see all

Anti-GOLPH3 antibody images

  • Lane 1 : Anti-GOLPH3 antibody (ab91492) at 0.5 µg/ml
    Lane 2 : Anti-GOLPH3 antibody (ab91492) at 1 µg/ml

    Lane 1 : rat lung tissue lysate
    Lane 2 : rat lung tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa,42 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • ab91492 staining GOLPH3 in human K562 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab91492 at a 1/150 dilution for 1 hour at 22°C. The secondary used was a DyLight 594 conjugated donkey anti-rabbit polyclonal used at a 1/200 dilution.

    Blue is nucleus staining by DAPI, red is GOLPH3.

    See Abreview

  • ab91492, staining GOLPH3 (red) in HCV-infected Huh 7.5.1 cells, by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed in 3% paraformaldehyde, permeabilized and blocked in antibody-binding buffer for 2 hours at 4°C. Samples were incubated with primary antibody and a fluorescence-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
  • ab91492, staining GOLPH3 in bovine aortic endothelial cells, by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100/PBS for 15 min at RT and blocked with 5% donkey serum/0.3% BSA/PBS for 30 min at RT. Samples were incubated with primary antibody at 1/100 dilution overnight at 4°C. An AlexaFlour®568-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody.
  • Immunofluorescence of GOLPH3 in Rat Lung cells using ab91492 at 20 ug/ml.

References for Anti-GOLPH3 antibody (ab91492)

This product has been referenced in:
  • Sangwung P  et al. Proteomic identification of S-nitrosylated Golgi proteins: new insights into endothelial cell regulation by eNOS-derived NO. PLoS One 7:e31564 (2012). ICC/IF ; Cow . Read more (PubMed: 22363674) »
  • Bishé B  et al. Role of Phosphatidylinositol 4-Phosphate (PI4P) and Its Binding Protein GOLPH3 in Hepatitis C Virus Secretion. J Biol Chem 287:27637-47 (2012). ICC/IF ; Human . Read more (PubMed: 22745132) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (K562)
Specification K562
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Mar 14 2012

DISCOUNT CODE: ***
Expiration date: July 13th, 2012

I am very pleased to hear you would like to accept our offer and test ab91492 in ICC/IF. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"