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Anti-GOLPH3 antibody images
Western blot - Anti-GOLPH3 antibody (ab98023)
All lanes : Anti-GOLPH3 antibody (ab98023) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Lung (Human) Tissue Lysate Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 4 : Mouse lung normal tissue lysate - total protein (ab29297) Lane 5 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 34 kDa Observed band size : 34 kDa Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 secondsThe predicted molecular weight of GOLPH3 is 34-kDa. We are unsure why we are seeing an additional band in HeLa and A549 whole cell lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Immunocytochemistry/ Immunofluorescence - Anti-GOLPH3 antibody (ab98023)This image is courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab98023 staining GOLPH3 in HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X100. Samples were incubated with ab98023 (1/200: in PBS) for 1 hours at 22°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody. The nuclei are counterstained with DAPI.
ICC/IF image of ab98023 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98023, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
References for Anti-GOLPH3 antibody (ab98023)
This product has been referenced in:
Guo YT et al. Correlational research of Golgi phosphorylation protein 3 expression in colorectal cancer. World J Gastroenterol21:13473-9 (2015).
Read more (PubMed: 26730158) »