Anti-GOLPH3 antibody - C-terminal (ab82297)

Overview

  • Product name
    Anti-GOLPH3 antibody - C-terminal
    See all GOLPH3 primary antibodies
  • Description
    Rabbit polyclonal to GOLPH3 - C-terminal
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C-terminal region of human GOLPH3.

  • Positive control
    • Mouse lung tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 34 kDa.
ELISA 1/1000.

Target

  • Function
    Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
  • Tissue specificity
    Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
  • Sequence similarities
    Belongs to the GOLPH3/VPS74 family.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coat protein antibody
    • Coat protein GPP34 antibody
    • FLJ90675 antibody
    • Golgi associated protein antibody
    • Golgi peripheral membrane protein 1, 34 kDa antibody
    • Golgi phosphoprotein 3 (coat protein) antibody
    • Golgi phosphoprotein 3 antibody
    • Golgi protein antibody
    • GOLP3_HUMAN antibody
    • Golph3 antibody
    • GOPP1 antibody
    • GPP34 antibody
    • MIDAS antibody
    • Mitochondrial DNA absence factor antibody
    see all

Images

  • Anti-GOLPH3 antibody - C-terminal (ab82297) at 1/50 dilution + mouse lung tissue lysate at 35 µg

    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,15 kDa,17 kDa,52 kDa. We are unsure as to the identity of these extra bands.

References

ab82297 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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