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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Ammonium Sulphate Precipitation
ab82297 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/100. Predicted molecular weight: 34 kDa.
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
Belongs to the GOLPH3/VPS74 family.
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
Information by UniProt
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
Anti-GOLPH3 antibody - C-terminal images
Western blot - GOLPH3 antibody - C-terminal (ab82297)
Anti-GOLPH3 antibody - C-terminal (ab82297) at 1/50 dilution + mouse lung tissue lysate at 35 µgPredicted band size :
34 kDaObserved band size :
30 kDa (why is the actual band size different from the predicted?
)Additional bands at :
100 kDa,15 kDa,17 kDa,52 kDa. We are unsure as to the identity of these extra bands.
References for Anti-GOLPH3 antibody - C-terminal (ab82297)
has not yet been referenced specifically in any publications.
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