The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPlays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
Sequence similaritiesBelongs to the GOLIM4 family.
Post-translational modificationsPhosphorylated probably by c-AMP-dependent kinases in its lumenal part. O-glycosylated; modified by sialic acid residues. N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues.
Cellular localizationGolgi apparatus > Golgi stack membrane. Endosome membrane. Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.