Overview

  • Product nameAnti-GORASP2 antibody
    See all GORASP2 primary antibodies
  • Description
    Mouse polyclonal to GORASP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length human GORASP2 protein (NP_056345.3, 1 a.a. ~ 452 a.a).

  • Positive control
    • mouse neural stem cell lysate, GORASP2 transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab68713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).

Target

  • FunctionMay be involved in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
  • Sequence similaritiesBelongs to the GORASP family.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications
    Myristoylated. Myristoylation is essential for the Golgi targeting.
    Palmitoylated.
    Phosphorylated in mitotic cells.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW552058 antibody
    • DKFZp434D156 antibody
    • FLJ13139 antibody
    • Golgi phosphoprotein 6 antibody
    • Golgi reassembly stacking protein 2 antibody
    • Golgi reassembly stacking protein 2, 55kDa antibody
    • Golgi reassembly stacking protein of 55 kDa antibody
    • Golgi reassembly-stacking protein 2 antibody
    • Golgi reassembly-stacking protein of 55 kDa antibody
    • GOLPH2 antibody
    • GOLPH6 antibody
    • GORASP2 antibody
    • GORS2_HUMAN antibody
    • GRASP55 antibody
    • GRS2 antibody
    • p59 antibody
    • RP23-428O15.1 antibody
    see all

Anti-GORASP2 antibody images

  • All lanes : Anti-GORASP2 antibody (ab68713) at 1/500 dilution

    Lane 1 : GORASP2 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 48 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • Anti-GORASP2 antibody (ab68713) at 1/500 dilution + neural stem cell lysate at 30 µg

    Secondary
    Peroxidase labeled horse anti-mouse IgG (H+L) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    This image is courtesy of an abreview submitted by Melina Reisenberg, King's College London, United Kingdom

    See Abreview

References for Anti-GORASP2 antibody (ab68713)

ab68713 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Neural stem cell line)
Loading amount 30 µg
Specification Neural stem cell line
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Melina Reisenberg

Verified customer

Submitted Feb 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"