Anti-GORASP2/GRASP55 antibody (ab68713)
Key features and details
- Mouse polyclonal to GORASP2/GRASP55
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GORASP2/GRASP55 antibody
See all GORASP2/GRASP55 primary antibodies -
Description
Mouse polyclonal to GORASP2/GRASP55 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human GORASP2/GRASP55. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_056345.3, 1 a.a. ~ 452 a.a)
-
Positive control
- mouse neural stem cell lysate, GORASP2/GRASP55 transfected 293T lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa). |
Target
-
Function
May be involved in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA. -
Sequence similarities
Belongs to the GORASP family.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsMyristoylated. Myristoylation is essential for the Golgi targeting.
Palmitoylated.
Phosphorylated in mitotic cells. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
-
Database links
- Entrez Gene: 26003 Human
- Omim: 608693 Human
- SwissProt: Q9H8Y8 Human
- Unigene: 431317 Human
-
Alternative names
- AW552058 antibody
- DKFZp434D156 antibody
- FLJ13139 antibody
see all
Images
-
All lanes : Anti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution
Lane 1 : GORASP2 / GRASP5 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 48 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Anti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution + neural stem cell lysate at 30 µg
Secondary
Peroxidase labeled horse anti-mouse IgG (H+L) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
Datasheets and documents
-
Datasheet download
References (1)
ab68713 has been referenced in 1 publication.
- Kim YH et al. Secretory autophagy machinery and vesicular trafficking are involved in HMGB1 secretion. Autophagy 17:2345-2362 (2021). PubMed: 33017561