Recombinant
RabMAb

Anti-GOSR1 antibody [EP1768Y] (ab53288)

Overview

  • Product name
    Anti-GOSR1 antibody [EP1768Y]
    See all GOSR1 primary antibodies
  • Description
    Rabbit monoclonal [EP1768Y] to GOSR1
  • Host species
    Rabbit
  • Specificity
    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications
    Suitable for: ICC/IF, WB, ICC, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GOSR1 (N terminal). The exact sequence is proprietary.

  • Positive control
    • SKBR-3 cell lysate 3T3 cell lysate HeLa cells Human stomach carcinoma
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab53288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/150.

For unpurified use at 1/50 - 1/100. 

WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

For unpurified use at 1/250 - 1/500. 

ICC 1/50 - 1/100.
Flow Cyt 1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/30.

IHC-P 1/100.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      GOSR1 is a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments; it is considered an essential component of the Golgi SNAP receptor (SNARE) complex.
    • Cellular localization
      Cell Membrane, Endoplasmic reticulum and Golgi Apparatus
    • Database links
    • Alternative names
      • 28 kDa cis Golgi SNARE p28 antibody
      • 28 kDa Golgi SNARE protein antibody
      • cis-golgi SNARE antibody
      • golgi integral membrane protein 2 antibody
      • Golgi SNAP receptor complex member 1 antibody
      • Golgi SNARE 28 kDa antibody
      • GOLIM2 antibody
      • GOS28 antibody
      • GOS28/P28 antibody
      • GOSR1 antibody
      • GS25 antibody
      • Gs28 antibody
      • P28 antibody
      see all

    Images

    • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GOSR1 with purified ab53288 at 1:120 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling GOSR1 with Purified ab53288 at 1:150 dilution. Cells were fixed in 100% Methanol. Cells were counterstained with None. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling GOSR1 with Purified ab53288 at 1:100 dilution (12.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • All lanes : Anti-GOSR1 antibody [EP1768Y] (ab53288) at 1/1000 dilution (purified)

      Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
      Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
      Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysates
      Lane 6 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
      Lane 7 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 29 kDa
      Observed band size: 29 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-GOSR1 antibody [EP1768Y] (ab53288) at 1/500 dilution (unpurified)

      Lane 1 : SKBR-3 cell lysate
      Lane 2 : 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 29 kDa
      Observed band size: 29 kDa

    • HeLa cells stained with unpurified ab53288 at 1/50 - 1/100 dilution

    • Human stomach carcinoma staining with unpurified ab53288 at 1/100 - 1/250 dilution

    References

    ab53288 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (HEK 293T cell transfected with pGOSR1-EGFP)
    Loading amount
    20 µg
    Specification
    HEK 293T cell transfected with pGOSR1-EGFP
    Gel Running Conditions
    Reduced Denaturing (4-15)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 25 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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