Anti-GOSR1 antibody [EP1768Y] (ab53288)

Overview

  • Product nameAnti-GOSR1 antibody [EP1768Y]
    See all GOSR1 primary antibodies
  • Description
    Rabbit monoclonal [EP1768Y] to GOSR1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human GOS-28.

  • Positive control
    • SKBR-3 cell lysate 3T3 cell lysate HeLa cells Human stomach carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab53288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/250 - 1/500. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IP 1/60.
ICC 1/50 - 1/100.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • RelevanceGOSR1 is a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments; it is considered an essential component of the Golgi SNAP receptor (SNARE) complex.
  • Cellular localizationCell Membrane, Endoplasmic reticulum and Golgi Apparatus
  • Database links
  • Alternative names
    • 28 kDa cis Golgi SNARE p28 antibody
    • 28 kDa Golgi SNARE protein antibody
    • cis-golgi SNARE antibody
    • golgi integral membrane protein 2 antibody
    • Golgi SNAP receptor complex member 1 antibody
    • Golgi SNARE 28 kDa antibody
    • GOLIM2 antibody
    • GOS28 antibody
    • GOS28/P28 antibody
    • GOSR1 antibody
    • GS25 antibody
    • Gs28 antibody
    • P28 antibody
    see all

Anti-GOSR1 antibody [EP1768Y] images

  • All lanes : Anti-GOSR1 antibody [EP1768Y] (ab53288) at 1/500 dilution

    Lane 1 : SKBR-3 cell lysate
    Lane 2 : 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • HeLa cells stained with ab53288 at 1/50 - 1/100 dilution
  • Human stomach carcinoma stainning with ab53288 at 1/100 - 1/250 dilution

References for Anti-GOSR1 antibody [EP1768Y] (ab53288)

ab53288 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (HEK 293T cell transfected with pGOSR1-EGFP)
Loading amount 20 µg
Specification HEK 293T cell transfected with pGOSR1-EGFP
Gel Running Conditions Reduced Denaturing (4-15)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Apr 25 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"