Overview

  • Product nameAnti-GOT2 antibody [13]
    See all GOT2 primary antibodies
  • Description
    Mouse monoclonal [13] to GOT2
  • Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    E.coli expressed recombinant protein corresponding to full length human GOT2

  • Positive control
    • Recombinant GOT2 protein

Properties

Applications

Our Abpromise guarantee covers the use of ab80223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 48 kDa.

Tested against recombiant fragment and Human cell lysate

ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.01-0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPlays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
  • Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localizationMitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AATM_HUMAN antibody
    • AL022787 antibody
    • Aspartate aminotransferase 2 antibody
    • Aspartate aminotransferase antibody
    • Aspartate aminotransferase, mitochondrial antibody
    • Aspartate transaminase 2 antibody
    • ASPATA antibody
    • EC 2.6.1.1 antibody
    • FABP 1 antibody
    • FABP pm antibody
    • FABP-1 antibody
    • FABPpm antibody
    • Fatty acid binding protein antibody
    • Fatty acid-binding protein antibody
    • FLJ40994 antibody
    • Glutamate oxaloacetate transaminase 2 antibody
    • Glutamate oxaloacetate transaminase 2, mitochondrial antibody
    • Glutamate oxaloacetate transaminase, mitochondrial antibody
    • Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) antibody
    • Got 2 antibody
    • GOT2 antibody
    • KAT4 antibody
    • KATIV antibody
    • kynurenine aminotransferase 4 antibody
    • Kynurenine aminotransferase IV antibody
    • kynurenine--oxoglutarate transaminase 4 antibody
    • kynurenine--oxoglutarate transaminase IV antibody
    • mAspAT antibody
    • MGC102129 antibody
    • MGC115763 antibody
    • mitAAT antibody
    • mitochondrial antibody
    • Mitochondrial aspartate aminotransferase antibody
    • OTTMUSP00000017748 antibody
    • Plasma membrane fatty acid binding protein antibody
    • Plasma membrane-associated fatty acid-binding protein antibody
    • Transaminase A antibody
    see all

Anti-GOT2 antibody [13] images

  • Overlay histogram showing HepG2 cells stained with ab80223 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80223, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-GOT2 antibody [13] (ab80223) at 1/1000 dilution + recombinant GOT2 protein

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-GOT2 antibody [13] (ab80223)

ab80223 has not yet been referenced specifically in any publications.

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