Overview

  • Product name
  • Description
    Rabbit polyclonal to GOT2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 174-392 of GOT2 (Uniprot ID: P00505).

  • Positive control
    • HepG2, Mouse Neuro2A and Rat lung whole cell lysates; HeLa cells; Human hepatoma, Rat RT2 xenograft and Human SkHep1 xenograft tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab153924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
  • Sequence similarities
    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization
    Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AATM_HUMAN antibody
    • AL022787 antibody
    • Aspartate aminotransferase 2 antibody
    • Aspartate aminotransferase antibody
    • Aspartate aminotransferase, mitochondrial antibody
    • Aspartate transaminase 2 antibody
    • ASPATA antibody
    • EC 2.6.1.1 antibody
    • FABP 1 antibody
    • FABP pm antibody
    • FABP-1 antibody
    • FABPpm antibody
    • Fatty acid binding protein antibody
    • Fatty acid-binding protein antibody
    • FLJ40994 antibody
    • Glutamate oxaloacetate transaminase 2 antibody
    • Glutamate oxaloacetate transaminase 2, mitochondrial antibody
    • Glutamate oxaloacetate transaminase, mitochondrial antibody
    • Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) antibody
    • Got 2 antibody
    • GOT2 antibody
    • KAT4 antibody
    • KATIV antibody
    • kynurenine aminotransferase 4 antibody
    • Kynurenine aminotransferase IV antibody
    • kynurenine--oxoglutarate transaminase 4 antibody
    • kynurenine--oxoglutarate transaminase IV antibody
    • mAspAT antibody
    • MGC102129 antibody
    • MGC115763 antibody
    • mitAAT antibody
    • mitochondrial antibody
    • Mitochondrial aspartate aminotransferase antibody
    • OTTMUSP00000017748 antibody
    • Plasma membrane fatty acid binding protein antibody
    • Plasma membrane-associated fatty acid-binding protein antibody
    • Transaminase A antibody
    see all

Images

  • Anti-GOT2 antibody (ab153924) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    10% SDS PAGE
  • Anti-GOT2 antibody (ab153924) at 1/1000 dilution + Mouse Neuro2A whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    10 % SDS-PAGE
  • Anti-GOT2 antibody (ab153924) at 1/1000 dilution + Rat lung lysate at 50 µg

    Predicted band size: 48 kDa



    10 % SDS-PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling GOT2 with ab153924 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Rat RT2 xenograft tissue, labeling GOT2 with ab153924 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human SKHep1 xenograft tissue, labeling GOT2 with ab153924 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue, labeling GOT2 with ab153924 at 1/500 dilution.

References

ab153924 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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