Overview

  • Product nameAnti-GPA33 antibody
    See all GPA33 primary antibodies
  • Description
    Mouse polyclonal to GPA33
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-319 of Human GPA33

  • Positive control
    • Human colon tissue lysate and 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects bands of approximately 50, 62 and 186 kDa (predicted molecular weight: 36 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role in cell-cell recognition and signaling.
    • Tissue specificityExpressed in normal gastrointestinal epithelium and in 95% of colon cancers.
    • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate.
      Palmitoylated.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • A33 antibody
      • Cell surface A33 antigen antibody
      • Cell surface A33 antigen precursor antibody
      • Glycoprotein A33 (transmembrane) antibody
      • Glycoprotein A33 antibody
      • Gpa33 antibody
      • GPA33_HUMAN antibody
      • MGC129986 antibody
      • MGC129987 antibody
      • OTTHUMP00000032346 antibody
      • Transmembrane glycoprotein A33 antibody
      see all

    Anti-GPA33 antibody images

    • Anti-GPA33 antibody (ab67102) at 1/500 dilution + human colon tissue lysate at 25 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 36 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-GPA33 antibody (ab67102) at 1/500 dilution

      Lane 1 : GPA33 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 36 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 186 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-GPA33 antibody (ab67102)

    ab67102 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. We do not know of any publications using ab67102 or ab108938. The protocol used with ab67102 is below: Blocking buffer (also Dilution buffer) Weigh non-fat milk 5 g and dissolve in 100 mL 1X PBST (0.2%) to a final mixture o...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"