Anti-GPA33 antibody [EPR4240] (ab108938)

Overview

  • Product nameAnti-GPA33 antibody [EPR4240]
    See all GPA33 primary antibodies
  • Description
    Rabbit monoclonal [EPR4240] to GPA33
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human GPA33.

  • Positive control
    • HT-29, Human small intestine, and Human fetal colon lysates. Human colon tissue. Human colonic adenocarcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionMay play a role in cell-cell recognition and signaling.
    • Tissue specificityExpressed in normal gastrointestinal epithelium and in 95% of colon cancers.
    • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate.
      Palmitoylated.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • A33 antibody
      • Cell surface A33 antigen antibody
      • Cell surface A33 antigen precursor antibody
      • Glycoprotein A33 (transmembrane) antibody
      • Glycoprotein A33 antibody
      • Gpa33 antibody
      • GPA33_HUMAN antibody
      • MGC129986 antibody
      • MGC129987 antibody
      • OTTHUMP00000032346 antibody
      • Transmembrane glycoprotein A33 antibody
      see all

    Anti-GPA33 antibody [EPR4240] images

    • All lanes : Anti-GPA33 antibody [EPR4240] (ab108938) at 1/1000 dilution

      Lane 1 : HT-29 lysate
      Lane 2 : Human small intestine lysate
      Lane 3 : Human fetal colon lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 36 kDa
    • ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • ab108938 showing negative staining in Breast carcinoma tissue.

    • ab108938 showing negative staining in Thyroid gland carcinoma tissue.

    • ab108938 showing positive staining in Ovarian carcinoma tissue.

    • ab108938 showing negative staining in Normal tonsil tissue.

    • ab108938 showing negative staining in Normal liver tissue.

    References for Anti-GPA33 antibody [EPR4240] (ab108938)

    ab108938 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. We do not know of any publications using ab67102 or ab108938. The protocol used with ab67102 is below: Blocking buffer (also Dilution buffer) Weigh non-fat milk 5 g and dissolve in 100 mL 1X PBST (0.2%) to a final mixture o...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"