Overview

  • Product nameAnti-GPAA1 antibody
    See all GPAA1 primary antibodies
  • Description
    Rabbit polyclonal to GPAA1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: LGSLFLWREL QEAPLSLAEG WQLFLAALAQ GVLEHHTYGA LLFPLLSLGL, corresponding to amino acids 558-607 of Human GPAA1

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately >70 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionEssential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate.
  • Tissue specificityUbiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues.
  • PathwayGlycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anchor attachment protein 1 (Gaa1p, yeast) homolog antibody
    • GAA1 antibody
    • GAA1 protein homolog antibody
    • Glycophosphatidylinositol anchor attachment 1 antibody
    • Glycosylphosphatidylinositol anchor attachment 1 protein antibody
    • Glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) antibody
    • GPAA 1 antibody
    • GPAA1 antibody
    • GPAA1_HUMAN antibody
    • GPAA1P anchor attachment protein 1 homolog antibody
    • GPI anchor attachment protein 1 antibody
    • GPI transamidase subunit antibody
    • hGAA1 antibody
    see all

Anti-GPAA1 antibody images

  • Anti-GPAA1 antibody (ab66032) at 0.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-rabbit IgG at 50,000 - 100,000 dilution

    Predicted band size : 68 kDa
    Observed band size : >70 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GPAA1 with ab66032 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-GPAA1 antibody (ab66032)

ab66032 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"