Overview

  • Product nameAnti-GPAM antibody
    See all GPAM primary antibodies
  • Description
    Rabbit polyclonal to GPAM
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 433-463 of Human GPAM (NP_065969.3).

  • Positive control
    • HepG2 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell line: HepG2

Properties

Applications

Our Abpromise guarantee covers the use of ab107506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 94 kDa.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionEsterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.
  • PathwayPhospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
  • Sequence similaritiesBelongs to the GPAT/DAPAT family.
  • DomainThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localizationMitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glycerol 3 phosphate acyltransferase 1 mitochondrial antibody
    • Glycerol 3 phosphate acyltransferase mitochondrial antibody
    • Glycerol-3-phosphate acyltransferase 1 antibody
    • Gpam antibody
    • GPAT antibody
    • GPAT-1 antibody
    • GPAT1 antibody
    • GPAT1_HUMAN antibody
    • KIAA1560 antibody
    • MGC26846 antibody
    • mitochondrial antibody
    • RP11-426E5.2 antibody
    see all

Anti-GPAM antibody images

  • ICC/IF image of ab107506 stained HepG2 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107506 at 1 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-GPAM antibody (ab107506) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size : 94 kDa

References for Anti-GPAM antibody (ab107506)

ab107506 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"