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Mouse polyclonal to GPAM
Full length GPAM (Human). (AAH30783.1).
- GPAM transfected 293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: PBS, pH 7.2
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Predicted molecular weight: 94 kDa.
Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Belongs to the GPAT/DAPAT family.
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Mitochondrion outer membrane.
Information by UniProt
- Glycerol 3 phosphate acyltransferase 1 mitochondrial antibody
- Glycerol 3 phosphate acyltransferase mitochondrial antibody
- Glycerol-3-phosphate acyltransferase 1 antibody
Western blot - Anti-GPAM antibody (ab68925)
All lanes :
Anti-GPAM antibody (ab68925) at 1/500 dilutionLane 1 :
GPAM transfected 293T cell lysateLane 2 :
Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.SecondaryAll lanes :
Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilutionPredicted band size:
94 kDaObserved band size:
98 kDa (why is the actual band size different from the predicted?
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"