Overview

  • Product name
  • Description
    Mouse polyclonal to GPAM
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length GPAM (Human). (AAH30783.1).

  • Positive control
    • GPAM transfected 293T cell lysate.

Applications

Our Abpromise guarantee covers the use of ab68925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 94 kDa.

Target

  • Function
    Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.
  • Pathway
    Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
  • Sequence similarities
    Belongs to the GPAT/DAPAT family.
  • Domain
    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization
    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glycerol 3 phosphate acyltransferase 1 mitochondrial antibody
    • Glycerol 3 phosphate acyltransferase mitochondrial antibody
    • Glycerol-3-phosphate acyltransferase 1 antibody
    • Gpam antibody
    • GPAT antibody
    • GPAT-1 antibody
    • GPAT1 antibody
    • GPAT1_HUMAN antibody
    • KIAA1560 antibody
    • MGC26846 antibody
    • mitochondrial antibody
    • RP11-426E5.2 antibody
    see all

Images

  • All lanes : Anti-GPAM antibody (ab68925) at 1/500 dilution

    Lane 1 : GPAM transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 94 kDa
    Observed band size : 98 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Zhang C  et al. Glycerolipid signals alter mTOR complex 2 (mTORC2) to diminish insulin signaling. Proc Natl Acad Sci U S A 109:1667-72 (2012). Read more (PubMed: 22307628) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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