Anti-GPAM antibody (ab69990)

Overview

  • Product name
  • Description
    Rabbit polyclonal to GPAM
  • Tested applications
    Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    15 amino acid peptide near the carboxy terminus of the human GPAM (GenBank accession no. NP_065969).

  • Positive control
    • WB: Rat brain tissue lysate. IHC-P: Mouse brain tissue.

Applications

Our Abpromise guarantee covers the use of ab69990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 94 kDa).
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function
    Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.
  • Pathway
    Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
  • Sequence similarities
    Belongs to the GPAT/DAPAT family.
  • Domain
    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization
    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glycerol 3 phosphate acyltransferase 1 mitochondrial antibody
    • Glycerol 3 phosphate acyltransferase mitochondrial antibody
    • Glycerol-3-phosphate acyltransferase 1 antibody
    • Gpam antibody
    • GPAT antibody
    • GPAT-1 antibody
    • GPAT1 antibody
    • GPAT1_HUMAN antibody
    • KIAA1560 antibody
    • MGC26846 antibody
    • mitochondrial antibody
    • RP11-426E5.2 antibody
    see all

Images

  • Paraffin-embedded mouse brain tissue stained for GPAM using ab69990 at 5 µg/ml in immunohistochemical analysis.

  • Lane 1 : Anti-GPAM antibody (ab69990) at 1 µg/ml
    Lane 2 : Anti-GPAM antibody (ab69990) at 2 µg/ml

    Lane 1 : rat brain tissue lysate
    Lane 2 : rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 94 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References

ab69990 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citratbuffer 0,1M pH-6
Permeabilization
Yes - Tween-80 in 1xPBS
Specification
Liver
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 05 2016

Vielen Dank für Ihre Email.

Leider sind seit der letzten Korrespondenz mehr als 6 Wochen vergangen, so dass wir die ungebrauchten Antikörper nicht mehr zurücknehmen können.

Unsere Rückgabefrist beträgt ei...

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Application
Western blot
Sample
Rabbit Purified protein (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted May 10 2012

Gracias por contactarnos.

Hemos recibido tu Abreview, y está en proceso de revisión. De hecho se os ha devuelto para que por favor añadáis información que falta a la imagen adjunta.

En cualquier ca...

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Gracias por tu respuesta.

Te indico el nuevo código para testar ab69990 en muestras de conejo mediante Western Blot: XXXXXXXX. Este caduca el día XX- XXXX-XXXX, y puede usarse una vez se publiquen los resultados en nuestra web. ...

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Te pido disculpas por la espera, y agradezco tu paciencia.

De los cuatro anticuerpos que te comenté en mi mail anterior, me han confirmado que de los inmunógenos usados para generarlos, sólo el de ab69990 comparte homolog...

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Gracias por contactarnos.

Tenemos cuatro anticuerpos contra GPAT1 en nuestro catálogo:

http://www.abcam.com/GPAM-antibody-ab69990.html

http://www.abcam.com/GPAM-antibody-ab117268.html

http://www.abcam.com/...

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Application
Western blot
Sample
Rabbit Cell lysate - whole cell (Liver)
Loading amount
30 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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