Anti-GPAM antibody (ab69990)


  • Product nameAnti-GPAM antibody
    See all GPAM primary antibodies
  • Description
    Rabbit polyclonal to GPAM
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    15 amino acid peptide near the carboxy terminus of the human GPAM (GenBank accession no. NP_065969).

  • Positive control
    • WB: Rat brain tissue lysate. IHC-P: Mouse brain tissue.


Our Abpromise guarantee covers the use of ab69990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 94 kDa).
IHC-P Use a concentration of 2.5 µg/ml.


  • FunctionEsterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.
  • PathwayPhospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
  • Sequence similaritiesBelongs to the GPAT/DAPAT family.
  • DomainThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localizationMitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glycerol 3 phosphate acyltransferase 1 mitochondrial antibody
    • Glycerol 3 phosphate acyltransferase mitochondrial antibody
    • Glycerol-3-phosphate acyltransferase 1 antibody
    • Gpam antibody
    • GPAT antibody
    • GPAT-1 antibody
    • GPAT1 antibody
    • GPAT1_HUMAN antibody
    • KIAA1560 antibody
    • MGC26846 antibody
    • mitochondrial antibody
    • RP11-426E5.2 antibody
    see all

Anti-GPAM antibody images

  • Paraffin-embedded mouse brain tissue stained for GPAM using ab69990 at 5 µg/ml in immunohistochemical analysis.

  • Lane 1 : Anti-GPAM antibody (ab69990) at 1 µg/ml
    Lane 2 : Anti-GPAM antibody (ab69990) at 2 µg/ml

    Lane 1 : rat brain tissue lysate
    Lane 2 : rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 94 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References for Anti-GPAM antibody (ab69990)

ab69990 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citratbuffer 0,1M pH-6
Permeabilization Yes - Tween-80 in 1xPBS
Specification Liver
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Jul 05 2016

Vielen Dank für Ihre Email.

Leider sind seit der letzten Korrespondenz mehr als 6 Wochen vergangen, so dass wir die ungebrauchten Antikörper nicht mehr zurücknehmen können.

Unsere Rückgabefrist beträgt ei...

Read More
Application Western blot
Sample Rabbit Purified protein (liver)
Loading amount 30 µg
Specification liver
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted May 10 2012

Gracias por contactarnos.

Hemos recibido tu Abreview, y está en proceso de revisión. De hecho se os ha devuelto para que por favor añadáis información que falta a la imagen adjunta.

En cualquier ca...

Read More

Gracias por tu respuesta.

Te indico el nuevo código para testar ab69990 en muestras de conejo mediante Western Blot: XXXXXXXX. Este caduca el día XX- XXXX-XXXX, y puede usarse una vez se publiquen los resultados en nuestra web. ...

Read More

Te pido disculpas por la espera, y agradezco tu paciencia.

De los cuatro anticuerpos que te comenté en mi mail anterior, me han confirmado que de los inmunógenos usados para generarlos, sólo el de ab69990 comparte homolog...

Read More

Gracias por contactarnos.

Tenemos cuatro anticuerpos contra GPAT1 en nuestro catálogo:

Read More
Application Western blot
Sample Rabbit Cell lysate - whole cell (Liver)
Loading amount 30 µg
Specification Liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 30 2009