Anti-GPAT-2 antibody (ab122373)
Key features and details
- Rabbit polyclonal to GPAT-2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GPAT-2 antibody -
Description
Rabbit polyclonal to GPAT-2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: HQKGVFLSQL LGEFSWLTEE ILLRGFDVGF SGQLRSLLQH SLSLLRAHVA LLRIRQGDLL VVPQPGPGLT HLA, corresponding to amino acids 474-540 of Human GPAT-2 (UniProt ID: Q6NUI2).
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Positive control
- IHC: Human pancreas tissue ICC/IF: human cell line BJ
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. -
Pathway
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. -
Sequence similarities
Belongs to the GPAT/DAPAT family. -
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 150763 Human
- SwissProt: Q6NUI2 Human
- Unigene: 348629 Human
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Alternative names
- Glycerol-3-phosphate acyltransferase 2 antibody
- GPAT-2 antibody
- GPAT2 antibody
see all
Images
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Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human heart muscle tissue labeling GPAT-2 with ab122373 at 1/200 dilution.
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Immunocytochemistry/immunofluorescence analysis of human cell line BJ labelling GPAT-2 with ab122373 at 2 µg/mL shows localization to mitochondria.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122373 has not yet been referenced specifically in any publications.