Overview

  • Product nameAnti-GPAT4 antibody
  • Description
    Rabbit polyclonal to GPAT4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human AGPAT6.

    (Peptide available as ab91636.)

  • Positive control
    • This antibody gave a positive signal in human skeletal muscle tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEsterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long-chain fatty acyl-CoAs.
    • Tissue specificityUbiquitous. Relatively high level of expression in skeletal muscle, heart and testis. Relatively low level of expression in lung.
    • PathwayPhospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
    • Sequence similaritiesBelongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
    • DomainThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
    • Cellular localizationEndoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 acyl sn glycerol 3 phosphate acyltransferase zeta antibody
      • 1 AGP acyltransferase 6 antibody
      • 1 AGPAT 6 antibody
      • 1-acylglycerol-3-phosphate O-acyltransferase 6 antibody
      • 1-AGP acyltransferase 6 antibody
      • 1-AGPAT 6 antibody
      • Acyl-CoA:glycerol-3-phosphate acyltransferase 4 antibody
      • AGPAT6 antibody
      • Glycerol-3-phosphate acyltransferase 4 antibody
      • GPAT4 antibody
      • GPAT4_HUMAN antibody
      • LPAAT zeta antibody
      • LPAAT-zeta antibody
      • Lysophosphatidic acid acyltransferase zeta antibody
      see all

    Anti-GPAT4 antibody images

    • Anti-GPAT4 antibody (ab76707) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 52 kDa
      Observed band size : 49 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 minutes

    References for Anti-GPAT4 antibody (ab76707)

    ab76707 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"