Overview

  • Product nameAnti-GPBB antibody [17B6]
    See all GPBB primary antibodies
  • Description
    Mouse monoclonal [17B6] to GPBB
  • SpecificityThis product reacts with BB and MM isoenzyme.
  • Tested applicationsSuitable for: ICC/IF, WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human).

  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
AP Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be used as Capture antibody. Can be paired for ELISA with Mouse monoclonal [10H5] to GPBB.

Target

  • FunctionPhosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
  • Sequence similaritiesBelongs to the glycogen phosphorylase family.
  • Post-translational
    modifications
    Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain glycogen phosphorylase antibody
    • Glycogen phosphorylase B antibody
    • Glycogen phosphorylase brain form antibody
    • Glycogen Phosphorylase Isoenzyme BB antibody
    • Glycogen phosphorylase, brain form antibody
    • MGC9213 antibody
    • Phosphorylase glycogen brain antibody
    • PYGB antibody
    • PYGB_HUMAN antibody
    see all

Anti-GPBB antibody [17B6] images

  • ICC/IF image of ab880 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab880, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GPBB antibody [17B6] (ab880)

ab880 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"