The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionFunctions as a GC-rich promoter-specific transactivating transcription factor.
Tissue specificityWidely expressed. Some isoforms may be specifically expressed in veins and arteries (at protein level). Isoform 4 is widely expressed. Isoform 1, isoform 2 and isoform 3 may be specifically expressed in vascular smooth muscle cells.
Sequence similaritiesBelongs to the vasculin family.
Cellular localizationNucleus. Cytoplasm. According to PubMed:12842993, it localizes to the cytoplasm.