Overview

  • Product nameAnti-GPCR C5L2 antibody
    See all GPCR C5L2 primary antibodies
  • Description
    Rabbit polyclonal to GPCR C5L2
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within the C terminal cytoplasmic domain of Human GPCR C5L2, conjugated to KLH.

  • Positive control
    • Human brain, cortex tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -80°C.
  • Storage bufferPreservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

  • FunctionReceptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, C4a and C3a and their desarginated derivatives. Couples weakly to G(i)-mediated signaling pathways.
  • Tissue specificityFrontal cortex, hippocampus, hypothalamus, pons and liver.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C5a anaphylatoxin chemotactic receptor C5L2 antibody
    • C5ARL_HUMAN antibody
    • C5L2 antibody
    • E030029A11Rik antibody
    • G protein coupled receptor 77 antibody
    • G protein coupled receptor C5L2 antibody
    • G-protein coupled receptor 77 antibody
    • GPF77 antibody
    • GPR77 antibody
    see all

Anti-GPCR C5L2 antibody images

  • Human brain, cortex (formalin fixed, paraffin embedded tissue) stained with ab116709 at 10 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-GPCR C5L2 antibody (ab116709)

ab116709 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Normal Human Bronchial Epithelial Cells)
Loading amount 80 µg
Specification Normal Human Bronchial Epithelial Cells
Gel Running Conditions Reduced Non-Denaturing (Native)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Feb 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"