Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR C5L2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Monkey
  • Immunogen

    Synthetic peptide, corresponding to a portion of the amino acids 1-50 of Human GPCR C5L2.

  • Positive control
    • Human brain (cerebellum) tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 0.2% Gelatin, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, C4a and C3a and their desarginated derivatives. Couples weakly to G(i)-mediated signaling pathways.
  • Tissue specificity
    Frontal cortex, hippocampus, hypothalamus, pons and liver.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C5a anaphylatoxin chemotactic receptor C5L2 antibody
    • C5ARL_HUMAN antibody
    • C5L2 antibody
    • E030029A11Rik antibody
    • G protein coupled receptor 77 antibody
    • G protein coupled receptor C5L2 antibody
    • G-protein coupled receptor 77 antibody
    • GPF77 antibody
    • GPR77 antibody
    see all

Images

  • ab77982 (10 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cerebellum, followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.

References

This product has been referenced in:
  • Yuan J  et al. C5a and its receptors in human anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Arthritis Res Ther 14:R140 (2012). IHC . Read more (PubMed: 22691190) »

See 1 Publication for this product

Customer reviews and Q&As

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