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antigen sequence, corresponding to amino acids 308-355 (SKKFQARFRS SVAEILKYTL WEVSCSGTVS EQLRNSETKN LCLLETAQ) of Human GPCR GPR1.
Our Abpromise guarantee covers the use of ab121315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|