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Rabbit polyclonal to GPCR GPR101
Predicted to work with:
Synthesized peptide derived from C-terminal region of human GPCR GPR101.
Extracts from COS-7 cells; HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/500 - 1/1000. Predicted molecular weight: 57 kDa.
1/500 - 1/1000.
Belongs to the G-protein coupled receptor 1 family.
Information by UniProt
G protein-coupled receptor 101 antibody
Western blot - GPCR GPR101 antibody (ab75241)
All lanes : Anti-GPCR GPR101 antibody (ab75241) at 1/500 dilution Lane 1 : extracts from COS-7 cells Lane 2 : extracts from COS-7 cells with immunizing peptide at 5 µg Lysates/proteins at 15 µg per lane. Predicted band size : 57 kDa Observed band size : 57 kDa
Immunocytochemistry/ Immunofluorescence - GPCR GPR101 antibody (ab75241)
Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75241. Right panel: same, after preincubation with the immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR101 antibody (ab75241)
IHC image of ab75241 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond
TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75241, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"