Overview

  • Product nameAnti-GPCR GPR101 antibody
    See all GPCR GPR101 primary antibodies
  • Description
    Rabbit polyclonal to GPCR GPR101
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from C-terminal region of human GPCR GPR101.

  • Positive control
    • Extracts from COS-7 cells; HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

Anti-GPCR GPR101 antibody images

  • All lanes : Anti-GPCR GPR101 antibody (ab75241) at 1/500 dilution

    Lane 1 : extracts from COS-7 cells
    Lane 2 : extracts from COS-7 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75241. Right panel: same, after preincubation with the immunizing peptide.
  • IHC image of ab75241 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75241, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-GPCR GPR101 antibody (ab75241)

ab75241 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab75241.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"