Anti-GPCR GPR116 antibody (ab111169)
Key features and details
- Rabbit polyclonal to GPCR GPR116
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GPCR GPR116 antibody
See all GPCR GPR116 primary antibodies -
Description
Rabbit polyclonal to GPCR GPR116 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide: V CYKHNFNA SS VSWCSKT VDV CCHFTN AANN SVWSP SMKLN LVPG ENITCQ, corresponding to amino acids 620-670 of Human GPCR GPR116
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Positive control
- Human breast carcinoma tissue; A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS (without Mg2+ and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab111169 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
Target
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Function
May have a role in the regulation of acid-base balance. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 1 GPS domain.
Contains 3 Ig-like (immunoglobulin-like) domains.
Contains 1 SEA domain. -
Post-translational
modificationsProteolytically cleaved into 2 highly conserved sites: one in the SEA domain and the other in the stalk domain region preceding the first transmembrane. The later 2 subunits, the extracellular subunit and the seven-transmembrane subunit, remain tightly associated and non-covalently linked. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 221395 Human
- SwissProt: Q8IZF2 Human
- Unigene: 362806 Human
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Alternative names
- DKFZp564O1923 antibody
- FLJ90640 antibody
- G protein coupled receptor 116 antibody
see all
Images
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ab111169 at 1/50 dilution staining GPCR GPR116 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with synthesized peptide.
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ab111169 at 1/100 dilution staining GPCR GPR116 in A549 cells by Immunofluorescence. The image on the right is treated with synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab111169 has been referenced in 3 publications.
- Georgiadi A et al. Orphan GPR116 mediates the insulin sensitizing effects of the hepatokine FNDC4 in adipose tissue. Nat Commun 12:2999 (2021). PubMed: 34016966
- Bouvier C et al. Recurrent novel THBS1-ADGRF5 gene fusion in a new tumor subtype "Acral FibroChondroMyxoid Tumors". Mod Pathol 33:1360-1368 (2020). PubMed: 32047233
- Tang X et al. GPR116, an adhesion G-protein-coupled receptor, promotes breast cancer metastasis via the Gaq-p63RhoGEF-Rho GTPase pathway. Cancer Res 73:6206-18 (2013). IHC ; Human . PubMed: 24008316