Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR116
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: V CYKHNFNA SS VSWCSKT VDV CCHFTN AANN SVWSP SMKLN LVPG ENITCQ, corresponding to amino acids 620-670 of Human GPCR GPR116

  • Positive control
    • Human breast carcinoma tissue; A549 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111169 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function
    May have a role in the regulation of acid-base balance.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 1 GPS domain.
    Contains 3 Ig-like (immunoglobulin-like) domains.
    Contains 1 SEA domain.
  • Post-translational
    modifications
    Proteolytically cleaved into 2 highly conserved sites: one in the SEA domain and the other in the stalk domain region preceding the first transmembrane. The later 2 subunits, the extracellular subunit and the seven-transmembrane subunit, remain tightly associated and non-covalently linked.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp564O1923 antibody
    • FLJ90640 antibody
    • G protein coupled receptor 116 antibody
    • GP116_HUMAN antibody
    • GPR116 antibody
    • Ig Hepta homolog antibody
    • KIAA0758 antibody
    • KPG_001 antibody
    • OTTHUMP00000016557 antibody
    • Probable G protein coupled receptor 116 antibody
    • Probable G-protein coupled receptor 116 antibody
    see all

Anti-GPCR GPR116 antibody images

  • ab111169 at 1/50 dilution staining GPCR GPR116 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with synthesized peptide.
  • ab111169 at 1/100 dilution staining GPCR GPR116 in A549 cells by Immunofluorescence. The image on the right is treated with synthesized peptide.

References for Anti-GPCR GPR116 antibody (ab111169)

This product has been referenced in:
  • Tang X  et al. GPR116, an adhesion G-protein-coupled receptor, promotes breast cancer metastasis via the Gaq-p63RhoGEF-Rho GTPase pathway. Cancer Res 73:6206-18 (2013). IHC ; Human . Read more (PubMed: 24008316) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse gene expression in HEK)
Permeabilization
Yes - .1% TritonX 100
Specification
mouse gene expression in HEK
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted May 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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