Anti-GPCR GPR119 antibody (ab73300)

Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR119
  • Specificity
    Reacts specifically with mouse 37 kDa protein.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from C terminal domain of mouse GPCR GPR119 protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 37 kDa.Can be blocked with GPCR GPR119 peptide (ab138476).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Receptor for the endogenous fatty-acid ethanolamide oleoylethanolamide (OEA) and lysophosphatidylcholine (LPC). Functions as a glucose-dependent insulinotropic receptor. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Seems to act through a G(s) mediated pathway.
  • Tissue specificity
    Predominantly expressed in the pancreas, especially in the islets.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor 119 antibody
    • G protein coupled receptor 2 antibody
    • G-protein coupled receptor 119 antibody
    • Glucose dependent insulinotropic receptor antibody
    • Glucose-dependent insulinotropic receptor antibody
    • GP119_HUMAN antibody
    • GPCR 2 antibody
    • GPCR2 antibody
    • GPR 119 antibody
    • Gpr119 antibody
    • HGPCR 2 antibody
    • HGPCR2 antibody
    • MGC119957 antibody
    • Seven transmembrane helix receptor antibody
    see all

References

ab73300 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you once again for your enquiry and for your patience. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

In answer to your question regarding blocknig peptide, we have now added this to the c...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Stomach)
Specification
Stomach
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Rodent Block M as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: RT°C
Username

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Verified customer

Submitted May 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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