Overview

  • Product nameAnti-GPCR GPR119 antibody
    See all GPCR GPR119 primary antibodies
  • Description
    Rabbit polyclonal to GPCR GPR119
  • Tested applicationsSuitable for: IHC-Fr, WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    KMEHAGAMAGGYRSPRTPSDFKALRTVSVL

    , corresponding to internal sequence amino acids 201-230 of Human GPCR GPR119

  • Positive control
    • WB: extracts from K562 cells ICC/IF: MCF-7 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/2000.
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/100.

Target

  • FunctionReceptor for the endogenous fatty-acid ethanolamide oleoylethanolamide (OEA) and lysophosphatidylcholine (LPC). Functions as a glucose-dependent insulinotropic receptor. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Seems to act through a G(s) mediated pathway.
  • Tissue specificityPredominantly expressed in the pancreas, especially in the islets.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor 119 antibody
    • G protein coupled receptor 2 antibody
    • G-protein coupled receptor 119 antibody
    • Glucose dependent insulinotropic receptor antibody
    • Glucose-dependent insulinotropic receptor antibody
    • GP119_HUMAN antibody
    • GPCR 2 antibody
    • GPCR2 antibody
    • GPR 119 antibody
    • Gpr119 antibody
    • HGPCR 2 antibody
    • HGPCR2 antibody
    • MGC119957 antibody
    • Seven transmembrane helix receptor antibody
    see all

Anti-GPCR GPR119 antibody images

  • All lanes : Anti-GPCR GPR119 antibody (ab75312) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from K562 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 28 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Left panel: immunofluorescence analysis of MCF-7 cells, using 1/500 ab75312. Right panel: same, after pre-incubation with the immunizing peptide.

References for Anti-GPCR GPR119 antibody (ab75312)

This product has been referenced in:
  • Little TJ  et al. Characterization of duodenal expression and localization of fatty acid-sensing receptors in humans: relationships with body mass index. Am J Physiol Gastrointest Liver Physiol 307:G958-67 (2014). Read more (PubMed: 25258406) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Stomach)
Specification Stomach
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Rodent Block M as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (gut)
Specification gut
Fixative Paraformaldehyde
Permeabilization Yes - Antigen retrieval
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Mar 14 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"