Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR125
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    TKRGNNTRTVDIVVLESSAQYCPPERV

    conjugated to KLH, corresponding to internal sequence amino acids 328-354 of Human GPCR GPR125

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
ELISA 1/5000.
IHC-Fr 1/100.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    GPR125 is an orphan receptor which has a leucine rich repeat (LRR),an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin andtitin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion,connective tissue, ear, embryo, eye,ganglion, heart, lung,placenta, and skin libraries.
  • Cellular localization
    Cell membrane; Multi pass membrane protein.
  • Database links
  • Alternative names
    • FLJ38547 antibody
    • G protein coupled receptor 125 antibody
    • PGR21 antibody
    • Probable G protein coupled receptor 125 precursor antibody
    • TEM5 like antibody
    • TEM5L antibody
    see all

Images

  • ICC/IF image of ab51705 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51705, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Shlush E  et al. In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells. Stem Cell Res Ther 8:37 (2017). Read more (PubMed: 28202061) »
  • Altman E  et al. Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues. Reproduction 148:417-27 (2014). Read more (PubMed: 25030892) »

See all 7 Publications for this product

Customer reviews and Q&As

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I have reviewed this case again, and it appears that WB was supposed to be removed as a tested application a while ago. However, this was impossible because of a programming error on ourinternal datasheetsi...

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Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell line(MDA-MB-231))
Loading amount
20 µg
Specification
breast cancer cell line(MDA-MB-231)
Gel Running Conditions
Reduced Denaturing (8% stacking gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Wang xiu

Verified customer

Submitted Jun 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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