Overview

  • Product name
  • Description
    Goat polyclonal to GPCR GPR125
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    CTNKSASSFKNS,

    corresponding to internal sequence amino acids 1098-1109 of human GPCR GPR125 (NP_660333.2)

  • Positive control
    • Human ovary lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:8,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 146 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      GPR125 is an orphan receptor which has a leucine rich repeat (LRR),an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin andtitin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion,connective tissue, ear, embryo, eye,ganglion, heart, lung,placenta, and skin libraries.
    • Cellular localization
      Cell membrane; Multi pass membrane protein.
    • Database links
    • Alternative names
      • FLJ38547 antibody
      • G protein coupled receptor 125 antibody
      • PGR21 antibody
      • Probable G protein coupled receptor 125 precursor antibody
      • TEM5 like antibody
      • TEM5L antibody
      see all

    Images

    • Anti-GPCR GPR125 antibody (ab82145) at 0.5 µg/ml + human ovary lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 146 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 hour

    References

    ab82145 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab82145.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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