Overview

  • Product name
  • Description
    Goat polyclonal to GPCR GPR125
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    CTNKSASSFKNS,

    corresponding to internal sequence amino acids 1098-1109 of human GPCR GPR125 (NP_660333.2)

  • Positive control
    • Human ovary lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:8,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 146 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      GPR125 is an orphan receptor which has a leucine rich repeat (LRR),an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin andtitin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion,connective tissue, ear, embryo, eye,ganglion, heart, lung,placenta, and skin libraries.
    • Cellular localization
      Cell membrane; Multi pass membrane protein.
    • Database links
    • Alternative names
      • FLJ38547 antibody
      • G protein coupled receptor 125 antibody
      • PGR21 antibody
      • Probable G protein coupled receptor 125 precursor antibody
      • TEM5 like antibody
      • TEM5L antibody
      see all

    Images

    • Anti-GPCR GPR125 antibody (ab82145) at 0.5 µg/ml + human ovary lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 146 kDa
      Observed band size: 140 kDa (why is the actual band size different from the predicted?)


      Exposure time: 1 hour


      An additional band of unknown identity was also consistently observed at 33 kDa. This band was successfully blocked by incubation with the immunizing peptide.

    References

    ab82145 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab82145.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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