Anti-GPCR GPR126 antibody (ab117092)

Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR126
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH, from the N terminal domain of Human GPCR GPR126.

  • Positive control
    • Human liver and prostate tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment with Proteinase K.

Target

  • Function
    Orphan receptor. May be required for normal differentiation of promyelinating Schwann cells and for normal myelination of axons. Signals probably through G-proteins to transiently elevate cAMP levels.
  • Tissue specificity
    Expressed in placenta and to a lower extent in pancreas and liver. Detected in aortic endothelial cells but not in skin microvascular endothelial cells.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 1 CUB domain.
    Contains 1 GPS domain.
    Contains 1 pentaxin domain.
  • Post-translational
    modifications
    Proteolytically cleaved into 2 conserved sites: one in the GPS domain (S1 site) and the other in the middle of the extracellular domain (S2 site). The proteolytic cleavage at S1 site generates an extracellular subunit and a seven-transmembrane subunit. Furin is involved in the cleavage of the S2 site generating a soluble fragment. Processing at the GPS domain occurred independent of and probably prior to the cleavage at the S2 site.
  • Cellular localization
    Cell membrane. Detected on the cell surface of activated but not resting umbilical vein.
  • Information by UniProt
  • Database links
  • Alternative names
    • adhesion G protein coupled receptor G6 antibody
    • APG1 antibody
    • Developmentally regulated G protein coupled receptor antibody
    • Developmentally regulated G-protein-coupled receptor antibody
    • dj287g14.2 antibody
    • DREG antibody
    • G protein coupled receptor 126 antibody
    • G-protein coupled receptor 126 antibody
    • GP126_HUMAN antibody
    • GPR126 antibody
    • HBV PreS1 transactivated protein 2 antibody
    • HBV PreS1-transactivated protein 2 antibody
    • LCCS9 antibody
    • OTTHUMP00000017320 antibody
    • OTTHUMP00000237093 antibody
    • OTTHUMP00000237094 antibody
    • OTTHUMP00000237115 antibody
    • PR126 antibody
    • Probable G protein coupled receptor 126 [Precursor] antibody
    • Probable G protein coupled receptor 126 antibody
    • Probable G-protein coupled receptor 126 [Precursor] antibody
    • Probable G-protein coupled receptor 126 antibody
    • PS1TP2 antibody
    • Vascular inducible G protein coupled receptor antibody
    • Vascular inducible G protein-coupled receptor antibody
    • VIGR antibody
    see all

Images

  • ab117092 at 10 µg/ml staining GPCR GPR126 in Formalin-Fixed, Paraffin-Embedded Human Liver tissue by Immunohistochemistry.
  • ab117092 at 10 µg/ml staining GPCR GPR126 in Formalin-Fixed, Paraffin-Embedded Human Prostate tissue by Immunohistochemistry.

References

ab117092 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO S169984-2
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Gentian Musa

Verified customer

Submitted Sep 16 2016

Thank you for contacting Abcam.

Below is a link that will give you a general antigen retrieval protocol, you may have to modify it to suit your own needs, but it will provide a good starting point:

http://ihcworld.com/_protocols/epi...

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Thank you for this feedback. I am sorry that I could not be of more help on this occasion. If you like to get some more information on our testing discount program, please find the Terms and Conditions of this offer at: www.abcam.com/collabora...

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Thank you for contacting us. I am happy to let you know the following immunogen sequences: ab65175: xxxxxxxxxxxxxxxxxx ab75356: yyyyyyyyyyyyyyyy In preliminary searches, I could not find conclusive matches for these peptides in zebrafish (onl...

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