Overview

  • Product name
  • Description
    Rabbit polyclonal to GPCR GPR14
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Non human primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GPCR GPR14.

    (Peptide available as ab92419.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: HeLa whole cell; Jurkat whole cell; HEK293 whole cell; mouse heart tissue; rat heart tissue; mouse eye tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab78449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 42 & 45 kDa (predicted molecular weight: 42 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • All lanes : Anti-GPCR GPR14 antibody (ab78449) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 42 kDa
      Observed band size : 42 kDa
      Additional bands at : 160 kDa,45 kDa (possible glycosylated form),65 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-GPCR GPR14 antibody (ab78449) at 1 µg/ml

      Lane 1 : Heart (Mouse) Tissue Lysate
      Lane 2 : Mouse eye tissue lysate - total protein (ab4029)
      Lane 3 : Heart (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 42 kDa
      Observed band size : 42 kDa
      Additional bands at : 20 kDa,32 kDa,90 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 4 minutes

    References

    ab78449 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab78449.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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